Microsatellite gene diversity analysis in anadromous arctic char, Salvelinus alpinus, from Labrador, Canada

نویسندگان

  • Louis Bernatchez
  • J. Brian Dempson
  • Sylvain Martin
چکیده

We analysed six loci among 257 Arctic char (Salvelinus alpinus) representing seven locations in Labrador and Newfoundland to provide a first assessment of microsatellites gene diversity in anadromous char and to determine the geographic scale of population structuring within the species. The number of alleles per locus varied between 9 and 48, and gene diversity ranged from 0.190 to 0.968. Significant FST and differences in allele frequencies were observed among most samples, as well as heterozygous deficiency, which was indicative of a Wahlund’s effect. These results implied the existence of genetically distinct populations on a microgeographic scale (less than 10 km) and that our samples represented an admixture of char from those populations that interchange among rivers for owerwintering, in congruence with tagging investigations. These results indicate that microsatellites potentially offer more sensitivity than allozymes and mitochondrial DNA to infer fine-scale population structure in anadromous arctic char. Résumé : Nous avons analysé six loci parmi 257 ombles chevalier (Salvelinus alpinus) provenant de sept sites au Labrador et à Terre-Neuve dans le but d’établir l’utilité des microsatellites pour la quantification du partage de variance génétique parmi les populations anadromes et pour documenter l’échelle de structuration génétique chez cette espèce. Les microsatellites se sont révélés modérément à hautement variables, avec un nombre d’allèles par locus variant entre 9 et 48 et une hétérozygotie attendue variant entre 0.190 et 0.968. Des valeurs de FST significatives, de même qu’une hétérogénéité des fréquences alléliques ont été observées entre la plupart des échantillons. Nous avons observé un déficit en hétérozygote associé à un effet Wahlund dans tous les échantillons, sauf un. Ces résultats traduisent l’existence de populations génétiquement différenciées sur une échelle microgéographique (inférieure à 10 km), de même qu’un mélange de ces populations dans les tributaires d’hivernage, corroborant ainsi les études de marquage. Nous concluons que les microsatellites offre une sensibilité supérieure aux marqueurs traditionnels pour l’étude de structures populationelles fines.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Microsatellite Gene Diversity Analysis in Landlocked Arctic Char from Maine

—Using six microsatellite loci, we characterized the 12 remaining populations of Arctic char Salvelinus alpinus naturally occurring in Maine. More specifically, we challenged the hypotheses based on previous analyses with other markers that (1) Arctic char from Floods Pond (known locally as silver char) represent a distinct evolutionary lineage and (2) all other Arctic char populations from Mai...

متن کامل

Genetic analysis of sympatric char populations in western Alaska: Arctic char (Salvelinus alpinus) and Dolly Varden (Salvelinus malma) are not two sides of the same coin.

The North Pacific Ocean has been of great significance to understanding biogeography and speciation in temperate faunas, including for two species of char (Salmonidae: Salvelinus) whose evolutionary relationship has been controversial. We examined the morphology and genetics (microsatellite and mitochondrial DNA) of Arctic char (Salvelinus alpinus) and Dolly Varden char (Salvelinus malma) in la...

متن کامل

Evidence for genetic distinction among sympatric ecotypes of Arctic char (Salvelinus alpinus) in south-western Alaskan lakes

Resource polymorphism may play an important role in the process of speciation. The Arctic char (Salvelinus alpinus) exhibits great phenotypic and genetic diversity across its range, making it an ideal species for studies of resource polymorphism and divergence. Here, we investigated genetic variation at 11 microsatellite loci among 287 Arctic char from five isolated yet proximate postglacial la...

متن کامل

Low levels of hybridization between sympatric Arctic char (Salvelinus alpinus) and Dolly Varden char (Salvelinus malma) highlights their genetic distinctiveness and ecological segregation

Understanding the extent of interspecific hybridization and how ecological segregation may influence hybridization requires comprehensively sampling different habitats over a range of life history stages. Arctic char (Salvelinus alpinus) and Dolly Varden (S. malma) are recently diverged salmonid fishes that come into contact in several areas of the North Pacific where they occasionally hybridiz...

متن کامل

Preference for nearshore and estuarine habitats in anadromous Arctic char (Salvelinus alpinus) from the Canadian high Arctic (Victoria Island, Nunavut) revealed by acoustic telemetry

Weused an array of fixed acoustic receivers (N = 42) to track the summermarinemovements of 121 anadromous Arctic char (Salvelinus alpinus) equipped with acoustic transmitters at three locations in the Cambridge Bay region, where commercial and subsistence fisheries target the species. The timing of transitions between salt and freshwaterwas influenced by the putative river of origin of tagged i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 1998